Biomedical

A multiplex immunoassay for human adipokine profiling

Type of publication: 
Matching Publication
Authors: 
H.S. Schipper, W. de Jager, M.E. van Dijk, J. Meerding, P.M.J. Zelissen, R.A. Adan, B.J. Prakken, E. Kalkhoven
Authors from the NMC: 
Published in: 
Clinical Chemistry
Date of publication: 
2010/08
Status of the publication: 
Published/accepted
Theme: 

BACKGROUND:

Adipose tissue secretory proteins, called adipokines, play pivotal roles in the pathophysiology of obesity and its associated disorders such as metabolic syndrome, type 2 diabetes, and cardiovascular disease. Because methods for comprehensive adipokine profiling in patient plasma and other biological samples are currently limited, we developed a multiplex immunoassay for rapid and high-throughput measurement of 25 adipokines in only 50 microL of sample.

Theme: 
Pages: 
2010; 56 (8): 1320-1328
DOI: 
10.1373/clinchem.2010.146118

Brown vs white adipocytes: the PPARgamma coregulator story

Type of publication: 
Matching Publication
Authors: 
A. Koppen, E. Kalkhoven
Authors from the NMC: 
Published in: 
FEBS Letters
Date of publication: 
2010/08
Status of the publication: 
Published/accepted
Theme: 

The development of adipose tissue is a process which involves the concerted cooperation of numerous transcription factors together with their coactivators and corepressors. The peroxisome proliferator-activated receptor gamma (PPARgamma) is considered to be one of the master regulators of adipocyte differentiation. The presence of two functionally distinct types of adipose tissue, white and brown (WAT and BAT), requires an even more complex regulation of adipose tissue development.

Theme: 
Pages: 
2010; 584 (15): 3250-3259
DOI: 
10.1016/j.febslet.2010.06.035

Central players in inherited lipodystrophies

Type of publication: 
Matching Publication
Authors: 
E.H. Jeninga, E. Kalkhoven
Authors from the NMC: 
Published in: 
Trends in Endocrinology and Metabolism
Date of publication: 
2010/10
Status of the publication: 
Published/accepted
Theme: 

Common obesity and inherited lipodystrophies, rare disorders characterized by a partial (familial partial lipodystrophy; FPLD) or complete (congenital generalized lipodystrophy; CGL) lack of adipose tissue, are both associated with metabolic complications such as insulin resistance and type 2 diabetes. Mutations in the transcription factor peroxisome proliferator activated receptor (PPAR)γ and a number of its downstream target genes result in lipodystrophy.

Theme: 
Pages: 
2010; 21 (10): 581-588
DOI: 
10.1016/j.tem.2010.06.006

The N-terminal fragment of human islet amyloid polypeptide is non-fibrillogenic in the presence of membranes and does not cause leakage of bilayers of physiologically relevant lipid composition

Type of publication: 
Matching Publication
Authors: 
L. Khemtémourian, M.F. Engel, R.M. Liskamp, J.W.M. Höppener, J.A. Killian
Authors from the NMC: 
Published in: 
Biochimica et Biophysica Acta
Date of publication: 
2010/09
Status of the publication: 
Published/accepted
Theme: 

Human islet amyloid polypeptide (hIAPP) forms amyloid fibrils in pancreatic islets of patients with type 2 diabetes mellitus (DM2). The formation of hIAPP fibrils has been shown to cause membrane damage which most likely is responsible for the death of pancreatic islet beta-cells during the pathogenesis of DM2. Previous studies have shown that the N-terminal part of hIAPP, hIAPP(1-19), plays a major role in the initial interaction of hIAPP with lipid membranes. However, the exact role of this N-terminal part of hIAPP in causing membrane damage is unknown.

Theme: 
Pages: 
2010; 1798 (9): 1805-1811
DOI: 
10.1016/j.bbamem.2010.05.022

The role of the disulfide bond in the interaction of islet amyloid polypeptide with membranes

Type of publication: 
Matching Publication
Authors: 
L. Khemtémourian, M.F. Engel, J.A. Kruijtzer, J.W.M. Höppener, R.M. Liskamp, J.A. Killian
Authors from the NMC: 
Published in: 
European Biophysics Journal
Date of publication: 
2010/08
Status of the publication: 
Published/accepted
Theme: 
Theme: 
Pages: 
2010; 39 (9): 1359-1364
DOI: 
10.1007/s00249-009-0572-4

Impaired processing of human pro-islet amyloid polypeptide is not a causative factor for fibril formation or membrane damage in vitro

Type of publication: 
Matching Publication
Authors: 
L. Khemtémourian, G. Lahoz Casarramona, D.P. Suylen, T.M. Hackeng, J.D. Meeldijk, B. de Kruijff, J.W.M. Höppener, J.A. Killian
Authors from the NMC: 
Published in: 
Biochemistry
Date of publication: 
2009/11
Status of the publication: 
Published/accepted
Theme: 
Theme: 
Pages: 
2009; 48 (46):1 0918-10925
DOI: 
10.1021/bi901076d

Quantitative proteomics and metabolomics analysis of normal human cerebrospinal fluid samples

Type of publication: 
Matching Publication
Authors: 
M.P. Stoop, L. Coulier, T. Rosenling, S. Shi, A.M. Smolinska, L. Buydens, K. Ampt, C. Stingl, A. Dane, B. Muilwijk, R. L. Luitwieler, P.A. Sillevis Smitt, R.Q. Hintzen, R. Bischoff, S.S. Wijmenga, T. Hankemeier, A.J. van Gool, T.M. Luider
Authors from the NMC: 
Published in: 
Molecular & Cellular Proteomics
Date of publication: 
2010/09
Status of the publication: 
Published/accepted
Theme: 

The analysis of cerebrospinal fluid (CSF) is used in biomarker discovery studies for various neurodegenerative central nervous system (CNS) disorders. However, little is known about variation of CSF proteins and metabolites between patients without neurological disorders. A baseline for a large number of CSF compounds appears to be lacking.

Theme: 
Pages: 
2010; 9 (9): 2063-2075
DOI: 
10.1074/mcp.M900877-MCP200

Hierarchical clustering analysis of blood plasma lipidomics profiles from mono- and dizygotic twin families

Type of publication: 
Matching Publication
Authors: 
H.H. Draisma, T.H. Reijmers, J.J. Meulman, J. van der Greef, T. Hankemeier, D.I. Boomsma
Published in: 
European Journal of Human Genetics
Date of publication: 
2013/01
Status of the publication: 
Published/accepted
Theme: 
Theme: 
Pages: 
2013; 21 (1): 95-101
DOI: 
10.1038/ejhg.2012.110

Purine pathway implicated in mechanism of resistance to aspirin therapy: pharmacometabolomics-informed pharmacogenomics

Type of publication: 
NMC Publication
Authors: 
L.M. Yerges-Armstrong, S. Ellero-Simatos, A. Georgiades, H. Zhu, J.P. Lewis, R.B. Horenstein, A.L. Beitelshees, A. Dane, T. Reijmers, T. Hankemeier, O. Fiehn, A.R. Shuldiner, R. Kaddurah-Daouk
Published in: 
Clinical Pharmacology and Therapeutics
Date of publication: 
2013/10
Status of the publication: 
Published/accepted
Theme: 

Although aspirin is a well-established antiplatelet agent, the mechanisms of aspirin resistance remain poorly understood. Metabolomics allows for measurement of hundreds of small molecules in biological samples, enabling detailed mapping of pathways involved in drug response. We defined the metabolic signature of aspirin exposure in subjects from the Heredity and Phenotype Intervention Heart Study. Many metabolites, including known aspirin catabolites, changed on exposure to aspirin, and pathway enrichment analysis identified purine metabolism as significantly affected by drug exposure.

Theme: 
Pages: 
2013; 94 (4): 525-532
DOI: 
10.1038/clpt.2013.119

Human metabolomics: strategies to understand biology

Type of publication: 
NMC Publication
Authors: 
R. Ramautar, R. Berger, J. van der Greef, T. Hankemeier
Published in: 
Current opinion in chemical biology
Date of publication: 
2013/07
Status of the publication: 
Published/accepted
Theme: 

Metabolomics provides a direct functional read-out of the physiological status of an organism and is in principle ideally suited to describe someone's health status. Whereas only a limited number of small metabolites are used in the clinics, in inborn errors of metabolism an extensive repertoire of metabolites are used as biomarkers. We discuss that the proper clinical phenotyping is crucial to find biomarkers and obtain biological insights for multifactorial diseases.

Theme: 
Pages: 
2013; pii: S1367-5931 (13) 00117-00118
DOI: 
10.1016/j.cbpa.2013.06.015