Quantitative Profiling
Elastomeric microvalves as tunable nanochannels for concentration polarization
Recent methodology in the phytochemical analysis of ginseng
This review summarises the most recent developments in ginseng analysis, in particular the novel approaches in sample pre-treatment and the use of high-performance liquid-chromatography-mass spectrometry. The review also presents novel data on analysing ginseng extracts by nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry (Fourier transform mass spectrometry) in the context of metabolomics.
The effect of preanalytical factors on stability of the proteome and selected metabolites in cerebrospinal fluid (CSF)
Metabolic profiling of ultrasmall sample volumes with GC/MS: from microliter to nanoliter samples
Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation
Microfluidic titer plate for stratified 3D cell culture
Insulin/IGF-1-mediated longevity is marked by reduced protein metabolism
Mutations in the daf-2 gene of the conserved Insulin/Insulin-like Growth Factor (IGF-1) pathway double the lifespan of the nematode Caenorhabditis elegans. This phenotype is completely suppressed by deletion of Forkhead transcription factor daf-16. To uncover regulatory mechanisms coordinating this extension of life, we employed a quantitative proteomics strategy with daf-2 mutants in comparison with N2 and daf-16; daf-2 double mutants.
Three-phase electroextraction: a new (online) sample purification and enrichment method for bioanalysis
The migration and at the same time enrichment of analytes from a liquid aqueous sample donor phase through an immiscible organic solvent layer acting as a filter
Pre-processing liquid chromatography/high-resolution mass spectrometry data: extracting pure mass spectra by deconvolution from the invariance of isotopic distribution
Mass spectra obtained by deconvolution of liquid chromatography/high-resolution mass spectrometry (LC/HRMS) data can be impaired by non-informative mass-over-charge (m/z) channels. This impairment of mass spectra can have significant negative influence on further post-processing, like quantification and identification.
A metric derived from the knowledge of errors in isotopic distribution patterns, and quality of the signal within a pre-defined mass chromatogram block, has been developed to pre-select all informative m/z channels.