RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model

A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C₈ column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R², 0.994−1.000), limit of detection (0.08−1.28 μg/mL plasma), repeatability (RSD, 2.7−7.9%) and intermediate precision (RSD, 2.7−15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.